Control of expression of the arginine deiminase operon of Streptococcus gordonii by CcpA and Flp.
نویسندگان
چکیده
In Streptococcus gordonii DL1, inactivation of the ccpA gene and a gene encoding an Fnr-like protein (Flp) demonstrated that CcpA was essential for carbohydrate catabolite repression and that Flp was required for optimal expression and anaerobic induction of the arginine deiminase system.
منابع مشابه
Environmental and growth phase regulation of the Streptococcus gordonii arginine deiminase genes.
A 1,026-bp open reading frame sharing significant similarity with queA, which encodes a predicted S-adenosylmethionine:tRNA ribosyltransferase-isomerase responsible for queosine modification of tRNAs, was found immediately 5' of the gene for the transcriptional activator (ArcR) of the arginine deiminase system (ADS) operon of Streptococcus gordonii. The role of QueA in bacterial physiology is e...
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Streptococcus suis is an important cause of infectious diseases in young pigs. Little is known about the virulence factors or protective antigens of S. suis. Recently, we have identified two proteins of the arginine deiminase system (ADS) of S. suis, which were temperature induced and expressed on the streptococcal surface (N. Winterhoff, R. Goethe, P. Gruening, M. Rohde, H. Kalisz, H. E. Smith...
متن کاملLoss of penicillin tolerance by inactivating the carbon catabolite repression determinant CcpA in Streptococcus gordonii.
OBJECTIVES Antibiotic tolerance is a phenomenon allowing bacteria to withstand drug-induced killing. Here, we studied a penicillin-tolerant mutant of Streptococcus gordonii (Tol1), which was shown to be deregulated in the expression of the arginine deiminase operon (arc). arc was not directly responsible for tolerance, but is controlled by the global regulator CcpA. Therefore, we sought whether...
متن کاملIsolation and molecular analysis of the gene cluster for the arginine deiminase system from Streptococcus gordonii DL1.
The arginine deiminase (AD) system (ADS) is one of two major ammonia-generating pathways in the oral cavity that play important roles in oral biofilm pH homeostasis and oral biofilm ecology. To initiate a study of the Streptococcus gordonii ADS, the ADS gene cluster was isolated from subgenomic DNA libraries of S. gordonii DL1 by using an arcB-specific probe. Nucleotide sequence analysis reveal...
متن کاملDeregulation of the arginine deiminase (arc) operon in penicillin-tolerant mutants of Streptococcus gordonii.
Penicillin tolerance is an incompletely understood phenomenon that allows bacteria to resist drug-induced killing. Tolerance was studied with independent Streptococcus gordonii mutants generated by cyclic exposure to 500 times the MIC of penicillin. Parent cultures lost 4 to 5 log(10) CFU/ml of viable counts/24 h. In contrast, each of four independent mutant cultures lost < or =2 log(10) CFU/ml...
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عنوان ژورنال:
- Journal of bacteriology
دوره 186 8 شماره
صفحات -
تاریخ انتشار 2004